Molecular and Phylogenetic Characteristics of Cytomegaloviruses Isolated from Children in Nizhny Novgorod
https://doi.org/10.35627/2219-5238/2021-337-4-25-30
Abstract
Introduction: Cytomegalovirus (CMV) infection is a serious problem of modern health care. It belongs to the category of socially significant infections and is characterized by polymorphism of clinical manifestations and high child mortality. Abroad, much attention is paid to virus genotyping, determining the role of various genotypes in the development of certain clinical forms of CMV infection, and developing a vaccine against congenital human cytomegalovirus infection. The objective of our study was to assess the genetic diversity of cytomegaloviruses in children of Nizhny Novgorod. Materials and methods: We analyzed clinical CMV isolates from body fluid samples (blood, urine, and saliva), viral DNA and its fragments in 580 children aged from 15 days to 16 years. Molecular biology (PCR, RTPCR, and sequencing), bioinformatics and statistical methods were applied in the study. Results: We established that CMV detection rates in children varied from 3.8 % to 18.9 % depending on the form of the disr ease. We assessed various method approaches to genotyping human cytomegalovirus clinical isolates, were first to determine prevalent gB1, gB2, and gN4a CMV genotypes in children in the Russian Federation, and revealed infected cases caused by two and three genotypes simultaneously. The phylogenetic analysis of UL55 and UL73 gene sequences indicates genetic diversity of Russian CMV isolates from children in the Nizhny Novgorod Region. Conclusions: New data on the prevalence of various CMV genotypes in children living in Nizhny Novgorod may be used in the system of epidemiological surveillance of cytomegalovirus infection while the results of genotyping and phylogenetic analysis of clinical CMV isolates may contribute to domestic vaccine development.
About the Authors
O. E. Vankova
Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology
Russian Federation
Russian Federation
Olga E. Vankova, Senior Researcher, Laboratory of Metagenomics and Molecular Indication of Pathogens
71 Malaya Yamskaya Street, Nizhny Novgorod, 603950
N. F. Brusnigina
Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology
Russian Federation
Russian Federation
Nina F. Brusnigina, Candidate of Medical Sciences, Associate Professor, Head of the Laboratory of Metagenomics and Molecular Indication of Pathogens
71 Malaya Yamskaya Street, Nizhny Novgorod, 603950
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Review
For citations:
Vankova O.E., Brusnigina N.F. Molecular and Phylogenetic Characteristics of Cytomegaloviruses Isolated from Children in Nizhny Novgorod. Public Health and Life Environment – PH&LE. 2021;(4):25-30. (In Russ.) https://doi.org/10.35627/2219-5238/2021-337-4-25-30
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