sredobЗдоровье населения и среда обитания – ЗНиСОPublic Health and Life Environment – PH&LE2219-52382619-0788ФБУЗ ФЦГиЭ Роспотребнадзора10.35627/2219-5238/2021-338-5-26-32sredob-524Research ArticleСтатьиНовые патогенные вирусы природных очагов Приморья, выделенные за 80­летнюю историю института (обзор вирусологических исследований)New pathogenic viruses of natural foci of Primorye isolated over the 80-year history of the Institute: A review of virological studieshttps://orcid.org/0000-0001-6387-1127ЛеоноваГ. Н.LeonovaG. N.

Леонова Галина Николаевна – д-р мед. наук., проф., глав. науч. сотр. лаборатории природно-очаговых трансмиссивных инфекций

ул. Сельская, д. 1, г. Владивосток, 690087

Galina N. Leonova, D.M.Sc., Professor, Chief Researcher, Laboratory of Natural Focal Transmissible Infections

1 Selskaya Street, Vladivostok 690087

galinaleon41@gmail.comhttps://orcid.org/0000-0001-7206-8299БеликовС. И.BelikovS. I.

Беликов Сергей Иванович – д-р биол. наук, проф., глав. науч. сотр.

ул. Улан-Баторская, 3, а/я 278, г. Иркутск, 664033

Sergei I. Belikov, D.Biol.Sc., Chief Researcher

3 Ulan-Batorskaya Street, PO Box 278, Irkutsk, 664033

sergeibelikov47@gmail.comhttps://orcid.org/0000-0002-2631-4724КондратовИ. Г.KondratovI. G.

Кондратов Илья Геннадьевич – канд. биол. наук, вед. науч. сотр.

ул. Улан-Баторская, 3, а/я 278, г. Иркутск, 664033

Ilya G. Kondratov, Candidate of Biological Sciences, Leading Researcher

3 Ulan-Batorskaya Street, PO Box 278, Irkutsk, 664033

igkondratov@rambler.ruФГБНУ «НИИ эпидемиологии и микробиологии имени Г. П. Сомова» РоспотребнадзораРоссияSomov Research Institute of Epidemiology and MicrobiologyRussian FederationФГБНУ Лимнологический институт РАНРоссияLimnological InstituteRussian Federation202108062021052632Copyright © Леонова Г.Н., Беликов С.И., Кондратов И.Г., 20212021Леонова Г.Н., Беликов С.И., Кондратов И.Г.Leonova G.N., Belikov S.I., Kondratov I.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zniso.fcgie.ru/jour/article/view/524

Введение. Открытие на Дальнем Востоке в 1937 г. нового вирусного заболевания – клещевого энцефалита (КЭ) – явилось началом таких исследований на Евразийском континенте. Цель исследования – провести вирусологический и эпидемиологический мониторинг клещевых вирусных инфекций в Приморском крае за 80­летний период. Материалы и методы. Выделено несколько сотен штаммов, принадлежащих к вирусам комплекса КЭ (семейство Flaviviridae) и к другим семействам, изучены их биологические, антигенные и молекулярно-генетические характеристики. Результаты. Наиболее полное представление о дальневосточной популяции вируса КЭ получено нами за последние десять лет. Оно основано на полногеномном секвенировании 50 штаммов, изолированных от пациентов с разными клиническими проявлениями инфекции, а также из иксодовых клещей. Установлено, что все штаммы принадлежат к одному дальневосточному субтипу вируса КЭ с тремя кластерами (Oshima-, Sofjin-, Senzhang-). Впервые на Евразийском континенте в 1972 году был выделен вирус Повассан из клещей Haemaphisalis longicornis. Филогенетический анализ на основе полногеномной характеристики штаммов Spassk-9, Nadezdinsk-1991 и Partizansk-2006, а также характеристики 5 фрагментов других штаммов вируса Повассан свидетельствовал о том, что все они относятся к генотипу I вируса Повассан. Впервые дано сравнительное описание трех штаммов Primorye-155-77, Primorye-20-79 и Primorye-185-91 вируса Louping ill. Комплексное изучение выделенного штамма Ozernoe при летальном исходе пациентки позволило впервые идентифицировать клинический случай лиссавирусной инфекции на азиатской территории России. Этот штамм генетически близок и имеет единого предка со штаммом Irkut и отнесен к возбудителям рода Lyssavirus, семейство Rhabdoviridae. Заключение. Выделение высокопатогенного лиссавируса, нескольких вирусов комплекса КЭ свидетельствует о том, что такой вирусологический надзор актуален и его следует продолжать.

Background: The discovery of a new viral disease called tick-borne encephalitis (TBE) in the Far East in 1937 triggered the thematic virology research on the Eurasian continent. The purpose of our study was to conduct a virological and epidemiological monitoring of tick-borne viral infections in the Primorsky Krai over an 80-year period. Materials and methods: Several hundreds of strains belonging to the viruses of the TBE complex (Flaviviridae family) and other families have been isolated; their biological, antigenic and molecular genetic characteristics have been studied. Results: The most complete picture of the Far Eastern population of the TBE virus was obtained in the 1990s based on whole genome sequencing of 50 TBEV strains isolated from patients with different clinical manifestations of the disease and from ixodid ticks. It was established that all the strains belong to the same Far Eastern subtype of TBEV with three clusters (Oshima-, Sofjin-, and Senzhang-). In 1972, the Powassan virus was first isolated from Haemaphisalis longicornis ticks on the Eurasian continent. Phylogenetic analysis based on the whole genome characteristics of the Spassk-9, Nadezdinsk-1991 and Partizansk-2006 strains, as well as the characteristics of five fragments of other Powassan virus strains, indicated that they all belong to the Powassan virus lineage I. The first comparative description of three strains Primorye-155-77, Primorye-20-79 and Primorye-185-91 of the Louping ill virus was given. A comprehensive study of the isolated Ozernoe strain of a deceased female patient enabled identification of the first clinical case of the lyssavirus disease in the Asian part of Russia. This strain is genetically close and has a common ancestor with the Irkut strain and is attributed to pathogens of the genus Lyssavirus, family Rhabdoviridae. Conclusion: Isolation of a highly pathogenic lyssavirus and several viruses of the TBE complex indicates the importance of such virology surveillance and proves the necessity of its continuation.

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The authors declare that there are no conflicts of interest present.