sredobЗдоровье населения и среда обитания – ЗНиСОPublic Health and Life Environment – PH&LE2219-52382619-0788ФБУЗ ФЦГиЭ Роспотребнадзора10.35627/2219-5238/2021-337-4-87-94sredob-508Research ArticleСтатьиМетоды исследования дендритных клеток человека, применимые для оценки действия вакцинMethods of Studying Human Dendritic Cells Applicable to Assessing Vaccine Efficacyhttps://orcid.org/0000-0003-1993-0622ТалаевВ. Ю.TalayevV. Yu.

Талаев Владимир Юрьевич – д.м.н., проф. заведующий лабораторией клеточной иммунологии

ул. Малая Ямская, д. 71, г. Нижний Новгород, 603950

Vladimir Yu. Talayev, D.M.Sc., Professor, Head of the Laboratory of Cellular Immunology

71 Malaya Yamskaya Street, Nizhny Novgorod, 603950

talaev@inbox.ruhttps://orcid.org/0000-0003-4097-6780СветловаМ. В.SvetlovaM. V.

Светлова Мария Владимировна – к.б.н., ст. науч. сотр.

ул. Малая Ямская, д. 71, г. Нижний Новгород, 603950

Maria V. Svetlova, Candidate of Biological Sciences, Senior Researcher, Laboratory of Cellular Immunology

71 Malaya Yamskaya Street, Nizhny Novgorod, 603950

https://orcid.org/0000-0001-5063-3111ЗаиченкоИ. Е.ZaichenkoI. Y.

Заиченко Ирина Евгеньевна – к.б.н., вед. науч. сотр.

ул. Малая Ямская, д. 71, г. Нижний Новгород, 603950

Irina Y. Zaichenko, Candidate of Biological Sciences, Leading Researcher, Laboratory of Cellular Immunology

71 Malaya Yamskaya Street, Nizhny Novgorod, 603950

https://orcid.org/0000-0003-4527-6134БабайкинаО. Н.BabaykinaO. N.

Бабайкина Ольга Николаевна – к.м.н., ст. науч. сотр.

ул. Малая Ямская, д. 71, г. Нижний Новгород, 603950

Olga N. Babaykina, Candidate of Medical Sciences, Senior Researcher, Laboratory of Cellular Immunology

71 Malaya Yamskaya Street, Nizhny Novgorod, 603950

https://orcid.org/0000-0003-1801-9693ВоронинаЕ. В.VoroninaE. V.

Воронина Елена Викторовна – к.б.н., науч. сотр.

ул. Малая Ямская, д. 71, г. Нижний Новгород, 603950

Elena V. Voronina, Candidate of Biological Sciences, Researcher, Laboratory of Cellular Immunology

71 Malaya Yamskaya Street, Nizhny Novgorod, 603950

ФБУН «Нижегородский научно-исследовательский институт эпидемиологии и микробиологии им. академика И.Н.Блохиной» РоспотребнадзораРоссияAcademician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and MicrobiologyRussian Federation202121052021048794Copyright © Талаев В.Ю., Светлова М.В., Заиченко И.Е., Бабайкина О.Н., Воронина Е.В., 20212021Талаев В.Ю., Светлова М.В., Заиченко И.Е., Бабайкина О.Н., Воронина Е.В.Talayev V.Y., Svetlova M.V., Zaichenko I.Y., Babaykina O.N., Voronina E.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zniso.fcgie.ru/jour/article/view/508

Введение. Вакцины являются одним из наиболее действенных средств профилактики инфекционных заболеваний. Их эффективность и безопасность гарантируется исследованиями свойств вакцин как на этапе их разработки, так и в ходе обязательных доклинических и клинических испытаний каждой новой вакцины. Дополнительную информацию о механизмах действия вакцин на клетки иммунной системы человека можно получить с использованием моделей иммунных реакций in vitro. Цель исследования – определить применимость отдельных методов исследования дендритных клеток человека in vitro для оценки действия вакцин. Дендритные клетки человека являются наиболее активными антигенпрезентирующими клетками, играющими ключевую роль в запуске первичного иммунного ответа на инфекцию или вакцину. Материалы и методы. Изучали влияние вакцин на созревание дендритных клеток, их фагоцитарную активность и способность стимулировать Т-лимфоциты in vitro. Результаты. Апробация методов проводилась с использованием широко применяемых вакцин с известным характером действия на иммунную систему. Показано, что все использованные вакцины индуцировали экспрессию маркеров созревания дендритных клеток. При этом различные вакцины индуцировали разный набор маркеров и степень экспрессии этих молекул. Описаны количественные методы оценки фагоцитоза и стимулирующей активности дендритных клеток. Выводы. Методы оценки фагоцитоза, фенотипического созревания и функциональных свойств дендритных клеток применимы для оценки действия вакцин. По нашему мнению, эти методы могут быть использованы для исследования механизмов действия прототипов вакцин на этапе их разработки и доклинических испытаний в качестве дополнения к традиционным способам оценки иммунного ответа. 

Introduction: Vaccines are one of the most effective means of preventing infectious diseases. Their effectiveness and safety are guaranteed by studies of vaccine properties, during their development and during the mandatory preclinical and clinical trials of each new vaccine. Additional information on the mechanisms of vaccine action on human immune system cells can be obtained using in vitro immune response models. The objective of the study was to determine applicability of certain methods of studying human dendritic cells in vitro to assessing the effect of vaccines. Dendritic cells are the most active antigen presenting cells, which play a key role in triggering a primary immune response to an infection or vaccine. Materials and methods: We studied the effect of vaccines on the maturation of dendritic cells, their phagocytic activity and the ability to stimulate T­lymphocytes in vitro. Results: To test the methods, we used vaccines with a known pattern of action on the immune system. All the vaccines induced the expression of dendritic cell maturation markers. At the same time, different vaccines induced a different set of markers and the degree of expression of these molecules. Quantitative methods for assessing phagocytosis and stimulating activity of dendritic cells are described. Conclusion: Methods for evaluation of phagocytosis, phenotypic maturation and functional properties of dendritic cells have been shown to be useful for evaluation of vaccine action. In our opinion, these methods, as a complement to traditional methods for evaluating the immune response, can be used to investigate the action of prototype vaccines at the stage of their development and preclinical trials.

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The authors declare that there are no conflicts of interest present.