sredobЗдоровье населения и среда обитания – ЗНиСОPublic Health and Life Environment – PH&LE2219-52382619-0788ФБУЗ ФЦГиЭ Роспотребнадзора10.35627/2219-5238/2021-337-4-25-30sredob-499Research ArticleСтатьиМолекулярная и филогенетическая характеристика изолятов цитомегаловируса, выделенных у детей Нижнего НовгородаMolecular and Phylogenetic Characteristics of Cytomegaloviruses Isolated from Children in Nizhny Novgorodhttps://orcid.org/0000-0002-9838-1133ВаньковаО. Е.VankovaO. E.

Ванькова Ольга Евгеньевна – ст. науч. сотр. лаборатории метагеномики и молекулярной индикации патогенов

ул. Малая Ямская, д. 71, г. Нижний Новгород, 603950

Olga E. Vankova, Senior Researcher, Laboratory of Metagenomics and Molecular Indication of Pathogens

71 Malaya Yamskaya Street, Nizhny Novgorod, 603950

voe0@mail.ruhttps://orcid.org/0000-0003-4582-5623БруснигинаН. Ф.BrusniginaN. F.

Бруснигина Нина Федоровна – к.м.н., доцент, заведующий лабораторией метагеномики и молекулярной индикации патогенов

ул. Малая Ямская, д. 71, г. Нижний Новгород, 603950

Nina F. Brusnigina, Candidate of Medical Sciences, Associate Professor, Head of the Laboratory of Metagenomics and Molecular Indication of Pathogens

71 Malaya Yamskaya Street, Nizhny Novgorod, 603950

nfbrusnigina@yandex.ruФБУН «Нижегородский научно-исследовательский институт эпидемиологии и микробиологии им. академика И.Н. Блохиной» РоспотребнадзораРоссияAcademician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and MicrobiologyRussian Federation202120052021042530Copyright © Ванькова О.Е., Бруснигина Н.Ф., 20212021Ванькова О.Е., Бруснигина Н.Ф.Vankova O.E., Brusnigina N.F.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zniso.fcgie.ru/jour/article/view/499

Введение. Цитомегаловирусная инфекция является одной из актуальных проблем здравоохранения, принадлежит к категории социально значимых инфекций, характеризуется полиморфизмом клиничес­ких проявлений и высокой смертностью детей раннего возраста. За рубежом большое внимание уделяется проблеме генотипирования вируса и определению роли различных генотипов в развитии определенных клинических форм цитомегаловирусной инфекции, активно ведутся работы по разработке вакцины. Цель работы – оценить генетическое разнообразие цитомегаловирусов, циркулирующих среди детей Нижнего Новгорода. Материалы и методы. Объектами исследования были клинические изоляты Сytomegalovirus, вы­деленные из образцов биологических субстратов (кровь, моча, слюна) у 580 детей в возрасте от 15 дней до 16 лет, ДНК и ее фрагменты. В работе использованы молекулярно­генетические (ПЦР, ПЦР РВ, секвениро­вание), биоинформационные и статистические методы. Результаты. Установлено, что показатели частоты обнаружения Сytomegalovirus у детей колебались в зависимости от нозологической формы заболевания от 3,8 % до 18,9 %. Проведена оценка различных методических подходов генотипирования клинических изо­лятов Сytomegalovirus. Впервые определены генотипы российских изолятов цитомегаловируса у детей, среди которых доминирующими оказались gB1, gB2, gN4а. Установлены случаи цитомегаловирусной инфекции, обусловленной одновременным присутствием двух и трех генотипов. Проведенный филогенетический ана­лиз нуклеотидных последовательностей генов UL55 и UL73 свидетельствует о генетической гетерогенности российских изолятов Сytomegalovirus, выделенных у детей Нижегородского региона. Полученные данные могут быть использованы в системе эпидемиологического надзора за цитомегаловирусной инфекцией. Выводы. Получены новые данные о распространенности различных геновариантов Сytomegalovirus среди детей Нижнего Новгорода. Результаты генотипирования и филогенетического анализа клинических изолятов Сytomegalovirus могут быть использованы для разработки отечественных вакцин.

Introduction: Cytomegalovirus (CMV) infection is a serious problem of modern health care. It belongs to the category of socially significant infections and is characterized by polymorphism of clinical manifestations and high child mortality. Abroad, much attention is paid to virus genotyping, determining the role of various genotypes in the development of certain clinical forms of CMV infection, and developing a vaccine against congenital human cytomegalovirus infection. The objective of our study was to assess the genetic diversity of cytomegaloviruses in children of Nizhny Novgorod. Materials and methods: We analyzed clinical CMV isolates from body fluid samples (blood, urine, and saliva), viral DNA and its fragments in 580 children aged from 15 days to 16 years. Molecular biology (PCR, RT­PCR, and sequencing), bioinformatics and statistical methods were applied in the study. Results: We established that CMV detection rates in children varied from 3.8 % to 18.9 % depending on the form of the dis­r ease. We assessed various method approaches to genotyping human cytomegalovirus clinical isolates, were first to determine prevalent gB1, gB2, and gN4a CMV genotypes in children in the Russian Federation, and revealed infected cases caused by two and three genotypes simultaneously. The phylogenetic analysis of UL55 and UL73 gene sequences indicates genetic diversity of Russian CMV isolates from children in the Nizhny Novgorod Region. Conclusions: New data on the prevalence of various CMV genotypes in children living in Nizhny Novgorod may be used in the system of epidemiological surveillance of cytomegalovirus infection while the results of genotyping and phylogenetic analysis of clinical CMV isolates may contribute to domestic vaccine development.

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The authors declare that there are no conflicts of interest present.