sredobЗдоровье населения и среда обитания – ЗНиСОPublic Health and Life Environment – PH&LE2219-52382619-0788ФБУЗ ФЦГиЭ Роспотребнадзора10.35627/2219-5238/2021-29-8-69-75sredob-20Research ArticleЭПИДЕМИОЛОГИЯEPIDEMIOLOGYСаногенетический эффект лимонной и янтарной кислот в условиях воздействия инактивированных M. tuberculosis на крысTherapeutic Effects of Citric and Succinic Acids in Rats Exposed to Inactivated M. tuberculosishttps://orcid.org/0000-0002-6233-5944СкупневскийС. В.SkupnevskiyS. V.

Скупневский Сергей Валерьевич – д-р биол. наук, ведущий научный сотрудник лаборатории субклеточных структур

ул. Пушкинская, д. 47, 362025, г. Владикавказ, Российская Федерация

Sergey V. Skupnevskiy, Dr. Sci. (Biol.), Leading Researcher, Laboratory of Subcellular Structures

47 Pushkinskaya Street, Vladikavkaz, RNO-Alania, 362025, Russian Federation

dreammas@yandex.ruhttps://orcid.org/0000-0001-9955-7447ТрухинаГ. М.TrukhinaG. M.

Трухина Галина Михайловна – д-р мед. наук, профессор, заведующая микробиологической лабораторией

ул. Семашко, д. 2, 141014, Московская обл., г.п. Мытищи, Российская Федерация

Galina M. Trukhina, Dr. Sci. (Med.), Prof., Head of Microbiological Laboratory

2 Semashko Street, Mytishchi, Moscow Region, 141014, Russian Federation

trukhina@list.ruhttps://orcid.org/0000-0002-0850-2537ПухаеваЕ. Г.PukhaevaE. G.

Пухаева Елена Георгиевна – младший научный сотрудник лаборатории субклеточных структур

ул. Пушкинская, д. 47, 362025, г. Владикавказ, Российская Федерация

Elena G. Pukhaeva, Junior Researcher, Laboratory of Subcellular Structures

47 Pushkinskaya Street, Vladikavkaz, RNO-Alania, 362025, Russian Federation

medgenetika435@yandex.ruhttps://orcid.org/0000-0001-5850-5173БадтиевА. К.BadtievA. K.

Бадтиев Алибек Кирилович – канд. биол. наук, научный сотрудник лаборатории субклеточных структур

ул. Пушкинская, д. 47, 362025, г. Владикавказ, Российская Федерация

Alibec K. Badtiev, Cand. Sci. (Biol.), Researcher, Laboratory of Subcellular Structures

47 Pushkinskaya Street, Vladikavkaz, RNO-Alania, 362025, Russian Federation

abadtiev@yandex.ruhttps://orcid.org/0000-0001-9424-0467РуруаФ. К.RuruaF. K.

Руруа Фатима Карловна – младший научный сотрудник лаборатории субклеточных структур

ул. Пушкинская, д. 47, 362025, г. Владикавказ, Российская Федерация

Fatima K. Rurua, Junior Researcher, Laboratory of Subcellular Structures

47 Pushkinskaya Street, Vladikavkaz, RNO-Alania, 362025, Russian Federation

medgenetika435@yandex.ruhttps://orcid.org/0000-0001-9105-0350БатаговаФ. Э.BatagovaF. E.

Батагова Фатима Эльбрусовна – младший научный сотрудник лаборатории субклеточных структур

ул. Пушкинская, д. 47, 362025, г. Владикавказ, Российская Федерация

Fatima E. Batagova, Junior Researcher, Laboratory of Subcellular Structures

47 Pushkinskaya Street, Vladikavkaz, RNO-Alania, 362025, Russian Federation

medgenetika435@yandex.ruhttps://orcid.org/0000-0002-9154-4729ФарниеваЖ. Г.FarnievaZ. G.

Фарниева Жанна Григорьевна – младший научный сотрудник лаборатории субклеточных структур

ул. Пушкинская, д. 47, 362025, г. Владикавказ, Российская Федерация

Zhanna G. Farnieva, Junior Researcher, Laboratory of Subcellular Structures

47 Pushkinskaya Street, Vladikavkaz, RNO-Alania, 362025, Russian Federation

medgenetika435@yandex.ruИнститут биомедицинских исследований – филиал ФГБУН Федерального научного центра «Владикавказский научный центр Российской академии наук»РоссияInstitute of Biomedical Research – Affiliate of Vladikavkaz Scientific Center of the RASRussian FederationФБУН «Федеральный научный центр гигиены им. Ф.Ф. Эрисмана» РоспотребнадзораРоссияF.F. Erisman Federal Scientific Center of HygieneRussian Federation2021180820212986975Copyright © Скупневский С.В., Трухина Г.М., Пухаева Е.Г., Бадтиев А.К., Руруа Ф.К., Батагова Ф.Э., Фарниева Ж.Г., 20212021Скупневский С.В., Трухина Г.М., Пухаева Е.Г., Бадтиев А.К., Руруа Ф.К., Батагова Ф.Э., Фарниева Ж.Г.Skupnevskiy S.V., Trukhina G.M., Pukhaeva E.G., Badtiev A.K., Rurua F.K., Batagova F.E., Farnieva Z.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zniso.fcgie.ru/jour/article/view/20Введение

Введение. Поиск способов коррекции патогенетических нарушений, обусловленных воздействием возбудителя социально значимого заболевания – M. tuberculosis на организм, определяет актуальность проведенного исследования и его

цель

цель: изучение роли лимонной и янтарной кислот в защитно-приспособительных процессах на фоне индуцированной инактивированными микобактериями патологии соединительной ткани у теплокровных животных.

Методы

Методы. Исследования проведены на крысах линии Wistar. На фоне индуцированной адъювантом Фрейнда (АФ, водно-масляная эмульсия термически обработанных микобактерий туберкулеза) патологии крысам вводили с едой смесь органических кислот в минимальной (17 мг/кг м.т.) и максимальной (88 мг/кг м.т.) дозировке на протяжении 4 недель. Гематологические и биохимические исследования проводили стандартными методами. Активность сукцинатдегидрогеназы (СДГ) в лимфоцитах крови определяли цитобиохимическим методом. Рентгеновские снимки получали на стационарном ветеринарном аппарате.

Результаты

Результаты. На фоне сформированной АФ патологии (лейкоцитоз (увеличение лейкоцитов на 28 % относительно негативного контроля, р < 0,05), окислительный стресс (рост содержания малонового диальдегида (МДА) на 40 %, p < 0,001; ингибирование каталазы на 4 %), субхондральный склероз головок костей), животные в условиях защитного воздействия карбоновыми кислотами характеризуются дозозависимым купированием иммунотоксических признаков заболевания (нормализация численности лейкоцитов (p < 0,05 относительно модельных животных); снижение МДА на 27 %, p < 0,001, активация каталазы на 10 %, p < 0,01; нормализация СДГ; снижение дистрофических изменений в суставном аппарате животных).

Заключение

Заключение. Результаты гематологических, биохимических и рентгенологических исследований свидетельствуют о возможности модификации цитрат-сукцинатной смесью патобиохимических и патоморфологических изменений, обусловленных введением инактивированных M. tuberculosis теплокровным животным, что позволяет глубже раскрыть патогенез и повысить эффективность проводимой терапии.

Introduction

Introduction. The search for methods of correcting pathogenetic disorders related to Mycobacterium tuberculosis, the causative agent of tuberculosis, a highly hazardous communicable and socially significant disease, determines the relevance of the research and its objective to study the role of citric and succinic acids in protective and adaptive processes in warm-blooded animals with connective tissue disorders induced by inactivated mycobacteria.

Materials and methods

Materials and methods. The study was conducted on male Wistar rats with diseases induced by complete Freund’s adjuvant (a mineral oil emulsion containing heat-killed Mycobacterium tuberculosis). The animals were given a feed-added mixture of organic acids at 17 mg/kg body weight (minimum) and 88 mg/kg body weight (maximum) for 4 weeks. Hematology and biochemistry tests were performed using standard methods. The activity of succinate dehydrogenase in blood lymphocytes was determined by the cytobiochemical method. X-rays were obtained using stationary veterinary imaging equipment.

Results

Results. The protective effect of carboxylic acids in the exposed animals with Freund’s adjuvant-induced leukocytosis (expressed by a 28 % increase in white blood cells compared to the negative control, p < 0.05), oxidative stress (expressed by an increase in the concentration of malondialdehyde (MDA) by 40 %, p < 0.001, and in inhibition of catalase by 4 %), and subchondral bone sclerosis was characterized by a dose-dependent reduction in immunotoxic manifestations of the disease such as normalization of the number of white blood cells (p < 0.05 compared to model animals); a 27 % reduction in MDA, p < 0.001, a 10 % catalase activation, p < 0.01; succinate dehydrogenase normalization, and a decrease in dystrophic changes in the articular system of animals.

Conclusion

Conclusion. The results of hematological, biochemical and radiological tests prove that pathological biochemical and morphological changes related to administration of inactivated M. tuberculosis to warm-blooded animals can be modified by a mixture of citric and succinic acids added to feed, which allows a better understanding of the pathogenesis and an increased therapy effectiveness.

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The authors declare that there are no conflicts of interest present.