sredobЗдоровье населения и среда обитания – ЗНиСОPublic Health and Life Environment – PH&LE2219-52382619-0788ФБУЗ ФЦГиЭ Роспотребнадзора2219-5238/2024-32-2-32-41sredob-1943Research ArticleКОММУНАЛЬНАЯ ГИГИЕНАCOMMUNAL HYGIENEМетодические подходы к определению антибиотиков в воде на уровне гигиенических нормативов методом высокоэффективной жидкостной хроматографии / масс-спектрометрииMethodological Approaches to Determination of Antibiotics in Water at the Level of Hygienic Standards Using High-Performance Liquid Chromatography–Mass Spectrometryhttps://orcid.org/0000-0002-2344-3037НурисламоваТ. В.NurislamovaT. V.

Нурисламова Татьяна Валентиновна – д.б.н., доцент, заведующая отделом химико-аналитических методов исследования.

ул. Монастырская, д. 82, Пермь, 614045

Tatyana V. Nurislamova - Dr. Sci. (Biol.), Assoc. Prof., Head of the Department of Analytical Chemistry, Federal Scientific Center for Medical and Preventive Health Risk Management Technologies.

82 Monastyrskaya Street, Perm, 614045

nurtat@fcrisk.ruhttps://orcid.org/0000-0001-6768-0045КарнажицкаяТ. Д.KarnazhitskayaT. D.

Карнажицкая Татьяна Дмитриевна – к.б.н., заведующая лабораторией методов жидкостной хроматографии.

ул. Монастырская, д. 82, Пермь, 614045

Tatyana D. Karnazhitskaya - Cand. Sci. (Biol.), Head of Liquid Chromatography Laboratory, Federal Scientific Center for Medical and Preventive Health Risk Management Technologies.

82 Monastyrskaya Street, Perm, 614045

tdkarn@fcrisk.ruhttps://orcid.org/0000-0002-3259-1509СтарчиковаМ. О.StarchikovaM. O.

Старчикова Мария Олеговна – младший научный сотрудник лаборатории методов жидкостной хроматографии.

ул. Монастырская, д. 82, Пермь, 614045

Maria O. Starchikova - Junior Researcher, Liquid Chromatography Laboratory, Federal Scientific Center for Medical and Preventive Health Risk Management Technologies.

82 Monastyrskaya Street, Perm, 614045

Starchikova.mar@yandex.ruТерентьевГ. И.TerentyevG. I.

Терентьев Геннадий Ильич – заведующий отделением физико-химических методов исследования ФБУЗ «Центр гигиены и эпидемиологии в Пермском крае».

ул. Монастырская, д. 82, Пермь, 614045; ул. Куйбышева, д. 50, Пермь, 614016

Gennady I. Terentyev - Head of the Department of Physicochemical Testing, Center for Hygiene and Epidemiology in the Perm Region.

82 Monastyrskaya Street, Perm, 614045; 50 Kuybyshev Street, Perm, 614016

ПоспеловаА. А.PospelovaA. A.

Поспелова Анна Анатольевна – к.фарм.н., химик-эксперт.

ул. Куйбышева, д. 50, Пермь, 614016

Anna A. Pospelova - Cand. Sci. (Pharm.), Expert Chemist, Center for Hygiene and Epidemiology in the Perm Region.

50 Kuybyshev Street, Perm, 614016

fbuz_cgepk@mail.ruФБУН «Федеральный научный центр медико-профилактических технологий управления рисками здоровью населения» РоспотребнадзораРоссияFederal Scientific Center for Medical and Preventive Health Risk Management TechnologiesRussian FederationФБУН «Федеральный научный центр медико-профилактических технологий управления рисками здоровью населения» Роспотребнадзора; ФБУЗ «Центр гигиены и эпидемиологии в Пермском крае»РоссияFederal Scientific Center for Medical and Preventive Health Risk Management Technologies; Center for Hygiene and Epidemiology in the Perm RegionRussian FederationФБУЗ «Центр гигиены и эпидемиологии в Пермском крае»РоссияCenter for Hygiene and Epidemiology in the Perm RegionRussian Federation2024290220243223241Copyright © Нурисламова Т.В., Карнажицкая Т.Д., Старчикова М.О., Терентьев Г.И., Поспелова А.А., 20242024Нурисламова Т.В., Карнажицкая Т.Д., Старчикова М.О., Терентьев Г.И., Поспелова А.А.Nurislamova T.V., Karnazhitskaya T.D., Starchikova M.O., Terentyev G.I., Pospelova A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://zniso.fcgie.ru/jour/article/view/1943Введение

Введение. Использование антибиотиков в медицине и ветеринарии привело к их накоплению в природной среде, в том числе в водных объектах питьевого назначения, и формированию устойчивости отдельных видов бактерий к действию антимикробных препаратов. Разработка методик анализа антибиотиков в водных средах актуальна для обеспечения контроля качества питьевой воды на уровне гигиенических нормативов, а также для изучения развития и распространения антибиотикорезистентности.

Цель исследования

Цель исследования: разработка методики определения антибиотиков классов макролидов, пенициллинов и фторхинолонов в воде на уровне гигиенических нормативов методом ВЭЖХ/МС-МС.

Материалы и методы

Материалы и методы. Исследования по разработке методики проведены методом ВЭЖХ/МС-МС на жидкостном хроматографе с масс-спектрометрическим детектором с тройным квадруполем. Извлечение антибиотиков из различных типов проб воды (водопроводной, природной) проводили методом твердофазной экстракции.

Результаты

Результаты. Разработана селективная и высокочувствительная методика определения 8 антибиотиков в воде. Степень экстракции аналитов из водных матриц составила 72–100 %, диапазон измеряемых концентраций на уровне 0,25–2,50 гигиенических нормативов при анализе проб воды объемом 10 см3, относительная погрешность определения антибиотиков в пробах воды без концентрирования – 20–24 %, с концентрированием на картриджах Oasis HLB – 24–34 %.

Обсуждение

Обсуждение. Рассмотрены методические подходы к разработке методики количественного определения антибиотиков групп пенициллинов, макролидов и хинолонов в воде методом ВЭЖХ/МС-МС с применением твердофазной экстракции в качестве пробоподготовки. Полученные результаты согласуются с данными научно-технической и методической литературы. Преимуществом разработанной методики является сокращение времени пробоподготовки, высокая чувствительность в сочетании с анализом незначительного объема образца исследуемой воды.

Заключение

Заключение. Разработанная методика может быть использована в гигиенических исследованиях содержания остаточных количеств антибиотиков в воде для оценки качества источников питьевого и культурно-бытового водоснабжения.

Introduction

Introduction: The use of antibiotics in medicine and veterinary medicine has led to their accumulation in the natural environment, including source water, and antimicrobial resistance of certain types of bacteria. The development of methods for analyzing antibiotics in aquatic environments is relevant for ensuring tap water quality control at the level of hygienic standards, as well as for studying the process of development and spread of antibiotic resistance.

The purpose of the study is to develop a method for determining such antibiotics as macrolides, penicillins, and fluoroquinolones in water at the level of hygienic standards using HPLC/MS-MS.

Materials and methods

Materials and methods: To elaborate the method, testing was done by HPLC/MS-MS using a liquid chromatograph with a triple quadrupole mass spectrometer. Extraction of antibiotics from various types of water samples (tap, natural) was carried out by solid-phase extraction.

Results

Results: We have developed a selective and highly sensitive method for the determination of eight antibiotics in water samples. The extraction efficiency for analytes ranged from 72 to 100 % and measured concentrations – from 0.25 to 2.50 of hygienic standards when analyzing 10 cm3 water samples; the relative error in determining antibiotics in water samples without concentration was 20–24 %, and 24–34 % in case of concentration on Oasis® HLB sorbent.

Discussion

Discussion: Approaches to developing a method for quantification of antibiotics of the penicillin, macrolide and quinolone classes in water by HPLC/MS-MS using solid-phase extraction for sample preparation are considered. The results are consistent with the data of scientific, technical and methodological literature. The advantages of this method include shorter sample preparation time, high sensitivity, and a small sample size.

Conclusion

Conclusion: Our method can be used in hygienic studies of residual amounts of antibiotics to assess source water quality.

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The authors declare that there are no conflicts of interest present.